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絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體

編號PAD559Hu01
物種Homo sapiens (Human，人)相同的名稱，不同的物種。
來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
標記物無標記物
免疫原RPD559Hu01-絲裂原激活蛋白激酶激酶1(MAP2K1)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500μg/mL
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。
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DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human MAP2K1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human MAP2K1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human MAP2K1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Thyroid cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human MAP2K1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human MAP2K1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human MAP2K1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant MAP2K1, Human.
Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Cerebrum lysate; Lane4: U937 cell lysate; Lane5: U87MG cell lysate; Lane6: HepG2 cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Human MAP2K1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

該抗體是針對MAP2K1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別MAP2K1。

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。

雜志	參考文獻
Graefe's Archive for Clinical and Experimental Ophthalmology	Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome[pubmed:27538909]
PLOS?ONE	Identification?of?Biomarkers?of?Impaired?Sensory?Profiles?among?Autistic?Patients.[pubmed:27824861]
Cancers	Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia[Pubmed: 31718075]
Cancers (Basel)	BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581[33540760]

編號	適用物種：Homo sapiens (Human，人)	應用(僅供研究使用，不用于臨床診斷！)
RPD559Hu01	絲裂原激活蛋白激酶激酶1(MAP2K1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAD559Hu01	絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體	WB; IHC; ICC; IP.
MAD559Hu21	絲裂原激活蛋白激酶激酶1(MAP2K1)單克隆抗體	WB; IHC; ICC; IP.
SED559Hu	絲裂原激活蛋白激酶激酶1(MAP2K1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD559Hu	絲裂原激活蛋白激酶激酶1(MAP2K1)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.