18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Cathelicidin Antimicrobial Peptide (CAMP) ,etc. by FLIA (Flow Luminescence Immunoassay)
CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMC419Ra
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時長1.5h
- 檢測范圍1.95-2000ng/mL
- 靈敏度最小可檢測劑量小于等于0.65 ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標(biāo)數(shù)7指標(biāo)數(shù)6指標(biāo)數(shù)5指標(biāo)數(shù)4指標(biāo)數(shù)3指標(biāo)數(shù)2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 87-96 | 91 |
| EDTA plasma(n=5) | 93-101 | 97 |
| heparin plasma(n=5) | 78-101 | 92 |
| sodium citrate plasma(n=5) | 88-96 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-105% | 80-98% | 85-93% | 83-91% |
| EDTA plasma(n=5) | 95-103% | 98-105% | 92-101% | 84-98% |
| heparin plasma(n=5) | 95-102% | 90-98% | 85-99% | 80-101% |
| sodium citrate plasma(n=5) | 99-105% | 97-105% | 97-105% | 81-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
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| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC419Ra01 | 18kDa陽離子抗菌肽(CAMP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC419Ra01 | 18kDa陽離子抗菌肽(CAMP)多克隆抗體 | WB; IHC; ICC; IP. |
| SEC419Ra | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEC419Ra | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC419Ra | 18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC419Ra11 | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Urology | Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells[PubMed: 25681250] |
| Sarcoidosis Vasc Diffuse Lung Dis | Cathelicidin as a link between sarcoidosis and tuberculosis[PubMed: 26422567] |
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| J Clin Microbiol. | Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.[Pubmed:27008883] |
| experimental dermatology | Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.[pubmed:27315464] |
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| Chinese Journal of Osteoporosis and Bone Mineral Research | Effects of cold exposure on the bone marrow adipose tissue[DOI: 10.3969/j.issn.1674-2591.2017.05.008] |
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| Experimental Parasitology | Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin[Pubmed: 30904694] |
| Theranostics | Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling[Pubmed: 31149039] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37[Pubmed: 32050475] |
| MICROB PATHOGENESIS | Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy[Pubmed: 32645421] |
| International Journal of Nanomedicine | Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide[Pubmed: 32606669] |
| 博士論文 | Rela??es causais entre a express?o dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com?…[] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies[33999928] |
| New Med | Analysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between??-[] |
| Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea[] | |
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