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雙調(diào)蛋白(AREG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Amphiregulin (AREG)

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

  • 雙調(diào)蛋白(AREG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 雙調(diào)蛋白(AREG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)雙調(diào)蛋白(AREG)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào)適用物種：Homo sapiens (Human，人)應(yīng)用(僅供研究使用，不用于臨床診斷！)
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參考文獻(xiàn)

雜志參考文獻(xiàn)
OnkologieAmphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological Characteristics[Karger: 315380]
Br J Cancer.Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer.[Pubmed:24800946]
Cancer SciAssociation between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancer[PubMed: 25707609]
J Cancer Res Clin OncolAmphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancer[PubMed: 26195282]
Oncotarget.?Serum?level?of?hepatocyte?growth?factor?is a?novel?marker?of?predicting?theoutcome?and?resistance?to the?treatment?with?trastuzumab?in HER2-positive patients with metastatic gastric cancer[Pubmed:26716644]
Acta?Pharmacologica SinicaAnti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi')[Pubmed: 19363516]
International Journal of Biological SciencesCD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17[]
NeoplasiaPatient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine[Pubmed: 33212364]
JOURNAL OF CELLULAR AND MOLECULAR MEDICINEPolarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets[Pubmed: 33164313]