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鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

  • 鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)鈣網(wǎng)蛋白(CRT)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種:Mus musculus (Mouse,小鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB486Mu02鈣網(wǎng)蛋白(CALR)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB486Mu01鈣網(wǎng)蛋白(CALR)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB486Mu01鈣網(wǎng)蛋白(CALR)多克隆抗體WB; IHC; ICC; IP.
PAB486Mu02鈣網(wǎng)蛋白(CALR)多克隆抗體WB; IHC; ICC; IP.
SEB486Mu鈣網(wǎng)蛋白(CALR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB486Mu鈣網(wǎng)蛋白(CALR)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSB486Mu01鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
Journal of Investigative DermatologyOxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes[Pubmed: 23771121]
International Journal of Molecular SciencesThe Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC[Mdpi: Source]
The Journal of Maternal-Fetal & Neonatal MedicineAmniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes[Pubmed:26953684]
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.[pubmed:28152632]
TalantaInvestigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling[Doi: 10.1016/j.talanta.2018.11.098]
Sci AdvThermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia[Pubmed:35171673]