補(bǔ)體成分3a(C3a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Complement Component 3a (C3a)
- 編號KSA387Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍78-5000pg/mL
- 可適靈敏度30pg/mL.
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對補(bǔ)體成分3a(C3a)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA387Hu01 | 補(bǔ)體成分3a(C3a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA387Hu01 | 補(bǔ)體成分3a(C3a)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA387Hu01 | 補(bǔ)體成分3a(C3a)多克隆抗體 | WB; IHC |
| MAA387Hu27 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu21 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu22 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu23 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu24 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu29 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu25 | 補(bǔ)體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA387Hu | 補(bǔ)體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA387Hu | 補(bǔ)體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA387Hu | 補(bǔ)體成分3a(C3a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA387Hu | 補(bǔ)體成分3a(C3a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA387Hu01 | 補(bǔ)體成分3a(C3a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 22 | Immobilization of soluble complement receptor 1 on islets.[ScienceDirect: S0142961211002596] |
| Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia[PubMed: 23415845] |
| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model[Pubmed: 23645587] |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats[Pubmed: 24353977] |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia?[PubMed: 26485247] |
| Am J Reprod Immunol | Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study[PubMed: 26388133] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis[Pubmed:27235358] |
| Plos One | Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice[Pubmed:26872035] |
| Digital Repository | Dosagem de fra??es ativadas do sistema complemento em empiema induzido em ratos[10183] |
| Colloids Surf B Biointerfaces. | Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns.[pubmed:28131092] |
| Pediatric Pulmonology | Accuracy of complement activation product levels to detect infected pleural effusion in rats[pubmed:28474458] |
| Journal of Immunology | The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression[Pubmed:29367209] |
| Artificial?Cells?Nanomedicine and Biotechnology Journal | Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly?…[Pubmed:29873524] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation[Doi: 10.1016/j.jcyt.2018.07.007] |
| Journal of?NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis[Pubmed: 30850976] |
| Clinical Immunology | Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss[] |
| Materials Science and Engineering: C | Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys[] |
| Nanomedicine | Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion[33549818] |
| EXPERIMENTAL EYE RESEARCH | Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression[33493474] |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease[Pubmed:35503088] |