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皮質(zhì)醇結(jié)合球蛋白(CBG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Corticosteroid Binding Globulin (CBG)

SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)

  • 皮質(zhì)醇結(jié)合球蛋白(CBG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 皮質(zhì)醇結(jié)合球蛋白(CBG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對皮質(zhì)醇結(jié)合球蛋白(CBG)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

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編號適用物種：Rattus norvegicus (Rat，大鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
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參考文獻(xiàn)

雜志參考文獻(xiàn)
General EndocrinologyThe Regulation of Rasd1 Expression by Glucocorticoids and Prolactin Controls Peripartum Maternal Insulin Secretion[Endjournals: Source]
PLoS OneModulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria Diet[PubMed: PMC3579843]
Annals of Anatomy Differences in colocalization of corticosteroid-binding globulin and glucocorticoid receptor immunoreactivity in the rat brain[ScienceDirect: S0940960212001628]
Physiological ReportsAdrenocortical control in the neonatal rat: ACTH‐and cAMP‐independent corticosterone production during hypoxia[Pubmed: 24303136]
Shock.Adrenocorticotropic Hormone But Not High-Density Lipoprotein Cholesterol or Salivary Cortisol Was a Predictor of Adrenal Insufficiency in Patients With Septic Shock.[Pubmed: 24667620]
Neurobiology of AgingGreater glucocorticoid receptor activation in hippocampus of aged rats sensitizes microglia[Pubmed:25559333]
Cell Death Differ. Novel p53 target genes secreted by the liver are involved in non-cell-autonomous reCavia (Guinea pig )lation[Pubmed:26358154]
Journal of Exercise, Sports & OrthopedicsImpact of Season and Oral Contraceptive use on Cortisol Levelsin Physically Active Women[exercise-sports-orthopedics:exercise-sports-orthop]
Impact of Season and Oral Contraceptive use on Cortisol Levels in Physically Active Women[diva2:1047030]
NeuropharmacologyImpaired glucocorticoid-mediated HPA axis negative feedback induced by juvenile social isolation in male rats[Pubmed:29407214]
Pharmacological ReportsThe impact of early-life stress on corticosteroid carrier protein levels and 11β-hydroxysteroid dehydrogenase 1 expression in adolescent rats[]