肌肌酸激酶(CKM)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Creatine Kinase, Muscle (CKM)
CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed
- 編號(hào)KSA109Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.56-100ng/mL
- 可適靈敏度0.62ng/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)肌肌酸激酶(CKM)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPA109Ra01 | 肌肌酸激酶(CKM)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA109Ra01 | 肌肌酸激酶(CKM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA109Ra02 | 肌肌酸激酶(CKM)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA109Ra01 | 肌肌酸激酶(CKM)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA109Ra71 | 肌肌酸激酶(CKM)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA109Ra21 | 肌肌酸激酶(CKM)單克隆抗體 | WB; IHC; ICC/IF |
| LAA109Ra72 | 肌肌酸激酶(CKM)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA109Ra | 肌肌酸激酶(CKM)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA109Ra | 肌肌酸激酶(CKM)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA109Ra | 肌肌酸激酶(CKM)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA109Ra01 | 肌肌酸激酶(CKM)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA109Ra01 | 肌肌酸激酶(CKM)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Inflammation | Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery[PubMed: 22583529] |
| PLoS One | Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility[PubMed: PMC3568149] |
| Kidney Int. | Fructokinase activity mediates dehydration-induced renal injury[Pubmed: 24336030] |
| Methylxanthines and cardiotoxicity | Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study[230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf] |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion[Pubmed:29393473] |
| Toxicological?Sciences | Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil[Pubmed: 30321427] |
| Clinical & Translational Oncology | The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells[Pubmed: 32447643] |