高爾基磷酸化蛋白2(GOLPH2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Golgi Phosphoprotein 2 (GOLPH2)
GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73
- 編號(hào)KSB668Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.94-60ng/mL
- 可適靈敏度0.37ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)高爾基蛋白73(GP73)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB668Mu02 | 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB668Mu02 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAB668Mu81 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB668Mu71 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB668Mu | 高爾基磷酸化蛋白2(GOLPH2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB668Mu | 高爾基磷酸化蛋白2(GOLPH2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular & Cellular Proteomics | LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues[Mcponline: 023507] |
| Afro-Egypt J Infect Endem Dis | Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma[Ajied: Ajied_System_Files] |
| Journal of Applied Pharmaceutical Science | Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients.[handle:123456789] |
| Oncology Letters | Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma[pubmed:29113278] |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study[Pubmed: 32198440] |
| Hepatobiliary & Pancreatic Diseases International | Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation[Pubmed: 32171652] |
| Biomolecules | Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-|êB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11??-[33540642] |
| ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[] |