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血幼素(HJV)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Hemojuvelin (HJV)

HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C

  • 血幼素(HJV)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 血幼素(HJV)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對血幼素 (HJV)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號適用物種:Mus musculus (Mouse,小鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB995Mu02血幼素(HJV)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB995Mu01血幼素(HJV)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB995Mu01血幼素(HJV)多克隆抗體WB; IHC; ICC; IP.
PAB995Mu02血幼素(HJV)多克隆抗體WB; IHC; ICC; IP.
SEB995Mu血幼素(HJV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB995Mu血幼素(HJV)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSB995Mu01血幼素(HJV)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
PLoS One.Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin Protein[PubMed: PMC3356351]
Journal ofHepcidin Levels Are Increased in Patients with Acute Ischemic Stroke: Preliminary Report[PubMed: 25881778]
European Journal of HaematologySoluble hemojuvelin in transfused and untransfused thalassaemic subjects[doi:10.1111]
American Journal of Nephrology The secret war against counterfeit science[pubmed:28492262]
Critical?CareUrinary biomarkers predict advanced acute kidney injury after cardiovascular surgery[Pubmed:29699579]
Scientific ReportsHemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery[Pubmed:29386545]
Journal of Cerebral Blood Flow and MetabolismThe functional role of hemojuvelin in acute ischemic stroke[Pubmed: 31307288]
Oxidative damage and iron dyshomeostasis in thalassemic patients[]
J Trace Elem Med Biol Caffeine and alcohol-friends or foes of human iron stores?[Pubmed:35063815]
Arch Pathol Lab MedUrinary Biomarkers Can Predict Weaning From Acute Dialysis Therapy in Critically Ill Patients[Pubmed:35311928]
Arch Pathol Lab MedThe Relationships among Plasma Fetuin-B, Thyroid Autoimmunity, and Fertilization Rate In Vitro Fertilization and Embryo Transfer[Pubmed:35340679]