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粘蛋白5B(MUC5B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)

MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin

  • 粘蛋白5B(MUC5B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 粘蛋白5B(MUC5B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)粘蛋白5B(MUC5B)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA684Mu02粘蛋白5B(MUC5B)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA684Mu01粘蛋白5B(MUC5B)活性蛋白Cell?culture;?Activity?Assays.
RPA684Mu01粘蛋白5B(MUC5B)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA684Mu01粘蛋白5B(MUC5B)多克隆抗體WB; IHC; ICC; IP.
PAA684Mu02粘蛋白5B(MUC5B)多克隆抗體WB; IHC; ICC; IP.
LAA684Mu81粘蛋白5B(MUC5B)多克隆抗體(異硫氰酸熒光素標(biāo)記)Applications: WB; IHC; ICC; IP.
LAA684Mu71粘蛋白5B(MUC5B)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
SEA684Mu粘蛋白5B(MUC5B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA684Mu粘蛋白5B(MUC5B)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA684Mu01粘蛋白5B(MUC5B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
The Journal of Biological ChemistryThe Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling[Jcb: 34199]
The Journal of Biological ChemistryThe ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling[Jcb: 247684]
Respiratory ResearhPyocyanin-induced mucin production is associated with redox modification of FOXA2[PubMed: PMC3765780]
RespirologySecreted mucins and airway bacterial colonization in non-CF bronchiectasis[PubMed: 26172851]
American Thoracic Society International Conference AbstractsMucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness[doi:10.1164]
Ann Am Thorac SocAirway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization[doi:10.1513]
Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой[10.20538/1682-0363-2017-2-146-158]
AllergyTh2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps[Pubmed:29802623]
European?Journal of Pharmacology?Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling[Pubmed:29382534]
Japan Society of Immunology & Allergology in Otolaryngologyインフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響[10.5648:jjiao.36.7]
Journal of Investigative?MedicineThe characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease[Pubmed: 30573494]
Mucosal ImmunologyASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism[Pubmed: 31278375]