對氧磷酶1(PON1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Paraoxonase 1 (PON1)
ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
- 編號KSA243Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍3.12-200ng/mL
- 可適靈敏度1.39ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對對氧磷酶1(PON1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA243Hu02 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA243Hu01 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA243Hu01 | 對氧磷酶1(PON1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA243Hu03 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA243Hu04 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA243Hu05 | 對氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA243Hu01 | 對氧磷酶1(PON1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA243Hu02 | 對氧磷酶1(PON1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA243Hu71 | 對氧磷酶1(PON1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA243Hu81 | 對氧磷酶1(PON1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA243Hu22 | 對氧磷酶1(PON1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA243Hu | 對氧磷酶1(PON1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA243Hu | 對氧磷酶1(PON1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA243Hu | 對氧磷酶1(PON1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA243Hu01 | 對氧磷酶1(PON1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population[pubmed:29254890] |
| Diabetes/Metabolism?Research?and Reviews | A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellitus[pubmed:29156090] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| Pharmacological Reports | The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder.[pubmed:28623707] |
| American Journal of Food Science and Health | Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats[ISSN: 2381-7224] |
| Metabolic brain disease | Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels[pubmed:28808839] |
| Therapeutic Apheresis And Dialysis | Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease[Pubmed:29368426] |
| Biological?Trace?Element?Research | Effect of Aqueous Extract of?Glycyrrhiza glabra?on the Biochemical Changes Induced by Cadmium Chloride in Rats[Pubmed: 30251228] |
| GLYCOCONJUGATE JOURNAL | Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer[Pubmed: 30607521] |
| Hematology. | Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails[Pubmed: 30661467] |
| Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy[Pubmed: 31162828] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605] |
| Nutrition, Metabolism and Cardiovascular Diseases | Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake[Pubmed: 31757567] |
| Environment International | Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins[Pubmed: 31783242] |
| RESPIRATORY RESEARCH | The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation?…[Pubmed: 33148285] |
| CLINICAL RHEUMATOLOGY | PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients[Pubmed: 32556934] |
| respiratory research | The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction[] |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans[33407555] |
| PLoS One | Vitamin D status and systemic redox biomarkers in adults with obesity[30973911] |
| Oxid Med Cell Longev | Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men[34917230] |