纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Plasminogen Activator Inhibitor 1 (PAI1)
SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor
- 編號KSA532Hu01
- 物種Homo sapiens (Human,人)相同的名稱,不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.5-800pg/mL
- 可適靈敏度5.1pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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特異性
試劑盒中的抗體對纖溶酶原激活物抑制因子1(PAI1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA532Hu02 | 纖溶酶原激活物抑制因子1(PAI1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA532Hu61 | 纖溶酶原激活物抑制因子1(PAI1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA532Hu01 | 纖溶酶原激活物抑制因子1(PAI1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA532Hu61 | 纖溶酶原激活物抑制因子1(PAI1)活性蛋白 | Cell?culture;?Activity?Assays. |
PAA532Hu01 | 纖溶酶原激活物抑制因子1(PAI1)多克隆抗體 | WB; IHC; ICC; IP. |
PAA532Hu02 | 纖溶酶原激活物抑制因子1(PAI1)多克隆抗體 | WB; IHC |
LAA532Hu71 | 纖溶酶原激活物抑制因子1(PAI1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA532Hu81 | 纖溶酶原激活物抑制因子1(PAI1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA532Hu22 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC; ICC/IF |
MAA532Hu24 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC |
MAA532Hu27 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | IHC |
MAA532Hu29 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA532Hu25 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA532Hu21 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC; ICC/IF |
MAA532Hu26 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA532Hu28 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC; ICC; IP. |
MAA532Hu23 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | WB; IHC |
FAA532Hu04 | 抗纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | Flow cytometry. |
FAA532Hu02 | 抗纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | Flow cytometry. |
FAA532Hu06 | 抗纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | Flow cytometry. |
FAA532Hu08 | 抗纖溶酶原激活物抑制因子1(PAI1)單克隆抗體 | Flow cytometry. |
LAA532Hu74 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA532Hu76 | 纖溶酶原激活物抑制因子1(PAI1)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MEA532Hu | 纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA532Hu | 纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA532Hu | 纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA532Hu | 纖溶酶原激活物抑制因子1(PAI1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA532Hu01 | 纖溶酶原激活物抑制因子1(PAI1)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA532Hu01 | 纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
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Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors[PubMed: 25546502] |
Archives of Physiology and Biochemistry | Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder[Pubmed:26934364] |
Naunyn-Schmiedeberg's Archives of Pharmacology | Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expression[pubmed:27447455] |
Environmental toxicology and pharmacology | Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats.[pubmed:28376377] |
Annals of Clinical & Laboratory Science | Alteration of Hemostatic Parameters in Patients with Different Levels of Subclinical Hypothyroidism and the Effect of L-thyroxine Treatment[pubmed:28249913] |
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Haematologica | Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain[Pubmed:29903766] |
Biomedicine & Pharmacotherapy | Puerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells[Pubmed:29775893] |
Archives of?Physical?Medicine?and Rehabilitation | Impact of Regular Physical Activity on Adipocytokines and Cardiovascular Characteristics in Spinal Cord–Injured Subjects[Pubmed:29548578] |
Marine Drugs | Anticoagulant and Antithrombotic Properties in Vitro and in Vivo of a Novel Sulfated Polysaccharide from Marine Green Alga Monostroma nitidum[Pubmed: 31027312] |
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Phytomedicine | Multi-omics analysis reveals the mechanisms of action and therapeutic regimens of traditional Chinese medicine, Bufei Jianpi granules: Implication for COPD drug?…[Pubmed:35121390] |