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纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Plasminogen Activator Inhibitor 1 (PAI1)

SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor

  • 纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對纖溶酶原激活物抑制因子1(PAI1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
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參考文獻(xiàn)

雜志參考文獻(xiàn)
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Archives of Physiology and BiochemistryActivation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder[Pubmed:26934364]
Naunyn-Schmiedeberg's Archives of PharmacologyCombined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expression[pubmed:27447455]
Environmental toxicology and pharmacologyCombined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats.[pubmed:28376377]
Annals of Clinical & Laboratory ScienceAlteration of Hemostatic Parameters in Patients with Different Levels of Subclinical Hypothyroidism and the Effect of L-thyroxine Treatment[pubmed:28249913]
Pharmacological reports Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independent[pubmed:28349880]
HaematologicaCytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain[Pubmed:29903766]
Biomedicine & PharmacotherapyPuerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells[Pubmed:29775893]
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