S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for S100 Calcium Binding Protein A8 (S100A8)
MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8
- 編號KSB792Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍7.81-500pg/mL
- 可適靈敏度3.1pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對S100鈣結(jié)合蛋白A8(S100A8)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB792Mu02 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB792Mu61 | S100鈣結(jié)合蛋白A8(S100A8)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB792Mu61 | S100鈣結(jié)合蛋白A8(S100A8)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC |
| PAB792Mu02 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB792Mu81 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB792Mu71 | S100鈣結(jié)合蛋白A8(S100A8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB792Mu21 | S100鈣結(jié)合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB792Mu | S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB792Mu | S100鈣結(jié)合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB792Mu01 | S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice[ScienceDirect: S0006291X1200352X] |
| American Journal of Reproductive Immunology | Association of Increased S100A8 Serum Protein with Early Pregnancy Loss[Pubmed:25252120] |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation[PubMed: 25934702] |
| J Immunol | S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet[PubMed: 26608915] |
| PLoS?One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation[Pubmed:26889680] |
| Journal?of?Alzheimer's?Disease | Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology.[pubmed:27911324] |
| ?Journal of Functional Foods | Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation[S1756464617300208] |
| International?Journal?of?Molecular?Medicine | Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells[pubmed:28498464] |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds.[pubmed:28672877] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis[10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis[10.1016:S0016-5085(18)33544-5] |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation[Pubmed:29496993] |
| European?review for?medical?and pharmacological?sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα.[Pubmed:29949169] |
| Foodborne Pathogens and Disease | Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model[Pubmed: 31116394] |
| Kidney International Reports | Systemic Inflammation Precedes Microalbuminuria in Diabetes[] |
| Cancer Management and Research | iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia[Pubmed: 31440093] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study[Pubmed: 31856598] |
| ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis[] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays[34012227] |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in[] |