轉(zhuǎn)鐵蛋白(TF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Transferrin (TF)
TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
- 編號(hào)KSC036Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.312-20ng/mL
- 可適靈敏度0.144ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)轉(zhuǎn)鐵蛋白(TRF)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC036Ra01 | 轉(zhuǎn)鐵蛋白(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC036Ra02 | 轉(zhuǎn)鐵蛋白(TF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC036Ra01 | 轉(zhuǎn)鐵蛋白(TF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC036Ra02 | 轉(zhuǎn)鐵蛋白(TF)多克隆抗體 | WB; IHC; ICC; IP. |
| FAC036Ra01 | 抗轉(zhuǎn)鐵蛋白(TF)多克隆抗體 | FCM |
| LAC036Ra81 | 轉(zhuǎn)鐵蛋白(TF)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAC036Ra71 | 轉(zhuǎn)鐵蛋白(TF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEC036Ra | 轉(zhuǎn)鐵蛋白(TF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC036Ra | 轉(zhuǎn)鐵蛋白(TF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC036Ra | 轉(zhuǎn)鐵蛋白(TF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC036Ra01 | 轉(zhuǎn)鐵蛋白(TF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance[PubMed: PMC3754973] |
| Physiological research | The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease[pubmed:27782743] |
| Journal of?Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia[Pubmed:29654921] |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients[Pubmed: 30486327] |
| The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria | Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria[] |
| Haematologica | New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia[Pubmed: 30792208] |