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腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)活性蛋白

Active Adenine Phosphoribosyltransferase (APRT)

AMP; APRTase

  • 腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)活性蛋白產(chǎn)品包裝(模擬)
  • 腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)活性蛋白產(chǎn)品包裝(模擬)
  • 腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)活性蛋白Gene sequencing
  • APC310Hu01.jpgSDS-PAGE圖
  • 腺嘌呤磷酸核糖轉(zhuǎn)移酶(APRT)活性蛋白Figure. Western Blot; Sample: Recombinant APRT, Human.
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

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