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骨成型蛋白4(BMP4)活性蛋白

Active Bone Morphogenetic Protein 4 (BMP4)

BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B

  • 骨成型蛋白4(BMP4)活性蛋白產品包裝(模擬)
  • 骨成型蛋白4(BMP4)活性蛋白產品包裝(模擬)
  • APA014Hu02.jpgFigure. SDS-PAGE
  • 骨成型蛋白4(BMP4)活性蛋白WB圖
  • Certificate通過ISO 9001、ISO 13485質量體系認證

活性實驗

BMP4 (Bone morphogenetic protein 4) is a member of the bone morphogenetic protein family, which is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been proven that HJV (Hemojuvelin) acts as a coreceptor of BMPs, including BMP4; therefore, a binding ELISA assay was constructed to detect the association of recombinant human BMP4 with recombinant human HJV. Briefly, BMP4 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HJV-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-BMP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of BMP4 with HJV was shown in Figure 1 and this effect was in a dose dependent manner.

To test the effect of BMP4 on cell apoptosis, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant human BMP4 diluted with 5% serum standard DMEM. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Apoptosis of HepG2 cells after incubation with BMP4 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human BMP4 for 72h. The result was shown in Figure 2. It was obvious that BMP4 significantly decreased cell viability of HepG2 cells. The ED50 of recombinant human BMP4 is 5.1 ug/ml.

Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant human BMP4

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當?shù)臈l件下存儲,損失率低于5%。

相關產品

編號適用物種:Homo sapiens (Human,人)應用(僅供研究使用,不用于臨床診斷!)
APA014Hu02骨成型蛋白4(BMP4)活性蛋白Cell?culture;?Activity?Assays.
APA014Hu01骨成型蛋白4(BMP4)活性蛋白Cell?culture;?Activity?Assays.
RPA014Hu02骨成型蛋白4(BMP4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA014Hu01骨成型蛋白4(BMP4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA014Hu61骨成型蛋白4(BMP4)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA014Hu01骨成型蛋白4(BMP4)多克隆抗體WB; IHC; ICC; IP.
PAA014Hu02骨成型蛋白4(BMP4)多克隆抗體WB; IHC; ICC; IP.
LAA014Hu71骨成型蛋白4(BMP4)多克隆抗體(生物素標記)WB; IHC; ICC.
LAA014Hu81骨成型蛋白4(BMP4)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
MAA014Hu22骨成型蛋白4(BMP4)單克隆抗體WB; IHC; ICC; IP.
SEA014Hu骨成型蛋白4(BMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA014Hu骨成型蛋白4(BMP4)檢測試劑盒(化學發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA014Hu骨成型蛋白4(BMP4)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA014Hu01骨成型蛋白4(BMP4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
Journal of Cellular BiochemistryTitanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway[PubMed: 26681207]
Domestic Animal EndocrinologyExpression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum[Pubmed:26945137]
FEBS?Open?BioBone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progression[Pubmed: 30972973]
Arch Gynecol ObstetThe relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese?…[34417839]