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碳酸酐酶Ⅸ(CA9)活性蛋白

Active Carbonic Anhydrase IX (CA9)

CA-IX; CAIX; MN; pMW1; P54/58N; Carbonic Dehydratase; Renal cell carcinoma-associated antigen G250; RCC-Associated Protein G250; Carbonate dehydratase IX

  • 碳酸酐酶Ⅸ(CA9)活性蛋白產(chǎn)品包裝(模擬)
  • 碳酸酐酶Ⅸ(CA9)活性蛋白產(chǎn)品包裝(模擬)
  • APD076Hu01.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Carbonic Anhydrase IX (CA9) also known as membrane antigen MN and renal cell carcinoma (RCC) associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. rhCA9 corresponds to the extracellular portion of human CA9. It catalyzes the reversible reaction of CO2 H2O = HCO3- H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. The recombinant human CA9 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of CA9 (diluted by Assay Buffer) to 50 μL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by deionized water). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

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編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
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