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心肌肌鈣蛋白I(cTnI)活性蛋白

Active Cardiac Troponin I (cTnI)

TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac

  • 心肌肌鈣蛋白I(cTnI)活性蛋白產(chǎn)品包裝(模擬)
  • 心肌肌鈣蛋白I(cTnI)活性蛋白產(chǎn)品包裝(模擬)
  • APA478Hu61.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Cardiac Troponin I (cTnI), as a cytoplasmic protein, is one of the most important regulatory proteins part of the troponin-tropomyosin complex localized on the actin (thin) myofilaments in cardiac myocytes.It is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.Besides,Actin Alpha 1, Cardiac Muscle (ACTC1) has been identified as an interactor of cTnI, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human cTnI and recombinant human ACTC1. Briefly, biotin-linked cTnI were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ACTC1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of cTnI and ACTC1 was shown in Figure 1, the EC50?for this effect is 0.066ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA478Hu02心肌肌鈣蛋白I(cTnI)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA478Hu61心肌肌鈣蛋白I(cTnI)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA478Hu01心肌肌鈣蛋白I(cTnI)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA478Hu01心肌肌鈣蛋白I(cTnI)活性蛋白Cell?culture;?Activity?Assays.
APA478Hu61心肌肌鈣蛋白I(cTnI)活性蛋白Cell?culture;?Activity?Assays.
PAA478Hu01心肌肌鈣蛋白I(cTnI)多克隆抗體WB; IHC
LAA478Hu71心肌肌鈣蛋白I(cTnI)多克隆抗體(生物素標(biāo)記)WB
MAA478Hu28心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC
RAA478Hu21心肌肌鈣蛋白I(cTnI)重組抗體WB
MAA478Hu21心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC
MAA478Hu27心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC
MAA478Hu23心肌肌鈣蛋白I(cTnI)單克隆抗體WB
MAA478Hu24心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC
MAA478Hu25心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC
MAA478Hu29心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC; ICC; IP.
MAA478Hu22心肌肌鈣蛋白I(cTnI)單克隆抗體WB; IHC; ICC; IP.
MAA478Hu26心肌肌鈣蛋白I(cTnI)單克隆抗體WB
FAA478Hu08抗心肌肌鈣蛋白I(cTnI)單克隆抗體FCM
FAA478Hu06抗心肌肌鈣蛋白I(cTnI)單克隆抗體Flow cytometry.
FAA478Hu04抗心肌肌鈣蛋白I(cTnI)單克隆抗體Flow cytometry.
FAA478Hu02抗心肌肌鈣蛋白I(cTnI)單克隆抗體Flow cytometry.
LAA478Hu74心肌肌鈣蛋白I(cTnI)單克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
SEA478Hu心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
HEA478Hu心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)Enzyme-linked immunosorbent assay for Antigen Detection.
AEA478Hu抗心肌肌鈣蛋白I抗體(Anti-cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antibody Detection.
SCA478Hu心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA478Hu心肌肌鈣蛋白I(cTnI)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA478Hu01心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

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