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組織蛋白酶G(CTSG)活性蛋白

Active Cathepsin G (CTSG)

CG; CTS-G

  • 組織蛋白酶G(CTSG)活性蛋白產(chǎn)品包裝(模擬)
  • 組織蛋白酶G(CTSG)活性蛋白產(chǎn)品包裝(模擬)
  • APD993Ra01.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Cathepsin G (CTSG) is a neutral proteinase secreted by neutrophil granulocytes. It belongs to the family of serine proteases. Cathepsin G is a glycoprotein, which contains 1% carbohydrate. The activity assay of recombinant rat CTSG was measured by its ability to cleave the peptide substrate Suc-Ala-Ala Pro-Phe-pNA. The reaction was performed in 160 mM Tris-HCl, 1.6 M NaCl, pH 7.4 (Assay Buffer). The CTSG was diluted to different concentrations and the reaction was initiated by adding 50 ul CTSG to 50 ul 1 mM substrate and then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat CTSG is >70 pmol/min/μg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號適用物種:Rattus norvegicus (Rat,大鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APD993Ra01組織蛋白酶G(CTSG)活性蛋白Cell?culture;?Activity?Assays.
RPD993Ra01組織蛋白酶G(CTSG)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAD993Ra01組織蛋白酶G(CTSG)多克隆抗體WB; IHC; ICC; IP.
MAD993Ra21組織蛋白酶G(CTSG)單克隆抗體WB; IHC; ICC; IP.
SED993Ra組織蛋白酶G(CTSG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMD993Ra組織蛋白酶G(CTSG)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志參考文獻(xiàn)
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.[pubmed:28152632]
LungMass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis[Pubmed: 31115649]