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矢車菊苷α2(CENTa2)活性蛋白

Active Centaurin Alpha 2 (CENTa2)

ADAP2; Cent-b; Arf-GAP With Dual PH Domain-Containing Protein 2

  • 矢車菊苷α2(CENTa2)活性蛋白產(chǎn)品包裝(模擬)
  • 矢車菊苷α2(CENTa2)活性蛋白產(chǎn)品包裝(模擬)
  • APF466Mu01.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Centaurin Alpha 2 (CENTa2) is a member of the Centaurin family of proteins, which are characterized by their unique structure and function in intracellular signaling and endosomal trafficking. It plays a role in various cellular processes by interacting with other molecules and is implicated in the regulation of cell growth, migration, and differentiation.
Besides,Neurofibromin 1 (NF1) has been identified as an interactor of CENTa2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CENTa2 and recombinant human NF1. Briefly, CENTa2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NF1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CENTa2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse CENTa2 and recombinant human NF1 was shown in Figure 1, the EC50?for this effect is 2.96ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號適用物種:Mus musculus (Mouse,小鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APF466Mu01矢車菊苷α2(CENTa2)活性蛋白Cell?culture;?Activity?Assays.
RPF466Mu01矢車菊苷α2(CENTa2)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAF466Mu01矢車菊苷α2(CENTa2)多克隆抗體WB; IHC; ICC; IP.