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凋亡相關因子(FAS)活性蛋白

Active Factor Related Apoptosis (FAS)

CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

  • 凋亡相關因子(FAS)活性蛋白產(chǎn)品包裝(模擬)
  • 凋亡相關因子(FAS)活性蛋白產(chǎn)品包裝(模擬)
  • 凋亡相關因子(FAS)活性蛋白Gene sequencing
  • APA030Hu03.jpgSDS-PAGE圖
  • 凋亡相關因子(FAS)活性蛋白Figure. Western Blot; Sample: Recombinant FAS, Human.
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。

相關產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應用(僅供研究使用,不用于臨床診斷!)
RPA030Hu02凋亡相關因子(FAS)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA030Hu03凋亡相關因子(FAS)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA030Hu01凋亡相關因子(FAS)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA030Hu03凋亡相關因子(FAS)活性蛋白Cell?culture;?Activity?Assays.
EPA030Hu61凋亡相關因子(FAS)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA030Hu61凋亡相關因子(FAS)活性蛋白Cell?culture;?Activity?Assays.
APA030Hu01凋亡相關因子(FAS)活性蛋白Cell?culture;?Activity?Assays.
PAA030Hu02凋亡相關因子(FAS)多克隆抗體WB; IHC
PAA030Hu03凋亡相關因子(FAS)多克隆抗體IHC; ICC/IF
PAA030Hu01凋亡相關因子(FAS)多克隆抗體WB; IHC; ICC; IP.
FAA030Hu01抗凋亡相關因子(FAS)多克隆抗體Flow cytometry.
LAA030Hu71凋亡相關因子(FAS)多克隆抗體(生物素標記)WB; IHC; ICC.
LAA030Hu81凋亡相關因子(FAS)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
FAA030Hu41抗凋亡相關因子(FAS)多克隆抗體(藻紅素標記)Flow cytometry.
FAA030Hu51抗凋亡相關因子(FAS)多克隆抗體(別藻藍蛋白標記)Flow cytometry.
FAA030Hu81抗凋亡相關因子(FAS)多克隆抗體(異硫氰酸熒光素標記)Flow cytometry.
MAA030Hu22凋亡相關因子(FAS)單克隆抗體WB; IHC; ICC; IP.
MAA030Hu21凋亡相關因子(FAS)單克隆抗體WB
SEA030Hu凋亡相關因子(FAS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA030Hu凋亡相關因子(FAS)檢測試劑盒(化學發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA030Hu凋亡相關因子(FAS)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA030Hu01凋亡相關因子(FAS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
European Journal of PharmacologyMontelukast abrogates rhabdomyolysis-induced acute renal failure via rectifying detrimental changes in antioxidant profile and systemic cytokines and apoptotic factors production [ScienceDirect: S0014299912002580]
J Sci Food Agric.Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet[Wiley: Source]
Tumor BiologyThe diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese women[Springer:Source]
Naunyn Schmiedebergs Arch PharmacolThe multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosis[PubMed: 26269412]
Pharmacological ReportsThe heat shock protein 90 inhibitor, 18-AAG, attenuates thioacetamide induced liver fibrosis in mice[Science: Article]
PLoS OneAronia melanocarpa?Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation[10.1371]
Molecular Diagnosis & TherapyThe Utility of Biomarkers in Osteoporosis Management[pubmed:28271451]
PLoS OneAronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation[pubmed:28081181]
Journal of Microbiology, Immunology and InfectionIdentification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients[pubmed:28655573]
Journal of Functional FoodsPolysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet[10.1016/j.jff.2017.07.020]
ToxicsZinc Oxide Nanoparticles Induced Oxidative DNA Damage, Inflammation and Apoptosis in Rat's Brain after Oral Exposure[Pubmed:29861430]
Indian?Journal?of?Medical?and?Paediatric?OncologyThe correlation between the level of doxorubicin-induced cardiac damage and serum soluble Fas in an experimental rat model[Doi: 10.4103/ijmpo.ijmpo_82_17]
Scientific ReportsEGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expression[Pubmed: 32703964]