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凝溶膠蛋白(GSN)活性蛋白

Active Gelsolin (GSN)

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

  • 凝溶膠蛋白(GSN)活性蛋白產(chǎn)品包裝(模擬)
  • 凝溶膠蛋白(GSN)活性蛋白產(chǎn)品包裝(模擬)
  • APA372Hu61.jpgFigure. SDS-PAGE
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Gelsolin (GSN), one of the most abundant actin-binding proteins, is a multifunctional regulator of physiological and pathological cellular process and regulate cell migration, cell morphology, proliferation and apoptosis. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm and a previous study revealed that the levels of GSN are decreased in various cancer. Profilin-1 (PFN1) can also bind to actin and affects the structure of the cytoskeleton. PFN1 is one of the ligands of GSN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GSN and recombinant human PFN1. Briefly, GSN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PFN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GSN and recombinant human PFN1 was shown in Figure 1, the EC50?for this effect is 1.11 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA372Hu02凝溶膠蛋白(GSN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu05凝溶膠蛋白(GSN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA372Hu61凝溶膠蛋白(GSN)活性蛋白Cell?culture;?Activity?Assays.
RPA372Hu06凝溶膠蛋白(GSN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu03凝溶膠蛋白(GSN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu04凝溶膠蛋白(GSN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu01凝溶膠蛋白(GSN)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA372Hu61凝溶膠蛋白(GSN)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CPA372Hu21凝溶膠蛋白(GSN)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
PAA372Hu05凝溶膠蛋白(GSN)多克隆抗體WB; IHC
PAA372Hu04凝溶膠蛋白(GSN)多克隆抗體WB; IHC; ICC; IP.
PAA372Hu06凝溶膠蛋白(GSN)多克隆抗體WB,IHC
PAA372Hu03凝溶膠蛋白(GSN)多克隆抗體WB; IHC; ICC; IP.
PAA372Hu02凝溶膠蛋白(GSN)多克隆抗體WB,IHC
PAA372Hu01凝溶膠蛋白(GSN)多克隆抗體WB; IHC; ICC; IP.
PAA372Hu08凝溶膠蛋白(GSN)多克隆抗體WB; IHC; ICC; IP.
LAA372Hu71凝溶膠蛋白(GSN)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
MAA372Hu27凝溶膠蛋白(GSN)單克隆抗體WB; IHC; ICC; IP.
MAA372Hu29凝溶膠蛋白(GSN)單克隆抗體WB; IHC; ICC; IP.
MAA372Hu22凝溶膠蛋白(GSN)單克隆抗體WB; IHC; ICC; IP.
MAA372Hu24凝溶膠蛋白(GSN)單克隆抗體WB; IHC
MAA372Hu21凝溶膠蛋白(GSN)單克隆抗體WB; IHC
MAA372Hu23凝溶膠蛋白(GSN)單克隆抗體WB; IHC
MAA372Hu25凝溶膠蛋白(GSN)單克隆抗體WB; IHC
MAA372Hu26凝溶膠蛋白(GSN)單克隆抗體WB; IHC; ICC; IP.
FAA372Hu02抗凝溶膠蛋白(GSN)單克隆抗體Flow cytometry.
FAA372Hu04抗凝溶膠蛋白(GSN)單克隆抗體Flow cytometry.
SEA372Hu凝溶膠蛋白(GSN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
WEA372Hu凝溶膠蛋白(GSN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA372Hu凝溶膠蛋白(GSN)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA372Hu凝溶膠蛋白(GSN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
PSA372Hu01凝溶膠蛋白(GSN)抗體對(duì)ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA372Hu01凝溶膠蛋白(GSN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
Neurochmical Research Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy[SpringerLink: 3585r8h46q7r0171]
Journal of NeuroinflammationTreatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury[BioMed: 17422094]
Biomedical Engineering and InformaticsApplication of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer[IEEE: 6098563]
European Journal of Obstetrics & Gynecology and Reproductive BiologyInvestigation of the actin scavenging system in pre-eclampsia[Pubmed: 24239294]
The Annals of Thoracic SurgeryProteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases[Pubmed: 24565402]
BMC Anesthesiol.?The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study[Pubmed:Pmc4132929]
Cell Biochemistry and BiophysicsGelsolin: Role of a Functional Protein in Mitigating Radiation Injury[Pubmed:25164111]
Neurosci LettPlasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease[PubMed: 25864780]
Proteomics Clinical ApplicationsComparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients[Pubmed:26680509]
PROTEOMICS?-?Clinical?ApplicationsComparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients.[pubmed:26680509]
Medicina Intensiva Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results.[pubmed:28365030]
Journal of?Alzheimers?DiseaseUrine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods[Pubmed:30040720]
World?Journal of PediatricsPlasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children[Pubmed:29427164]
BiomedicinesProduction and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various?…[Pubmed:35327525]