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鐵調(diào)素(Hepc)活性蛋白

Active Hepcidin (Hepc)

HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

  • 鐵調(diào)素(Hepc)活性蛋白產(chǎn)品包裝(模擬)
  • 鐵調(diào)素(Hepc)活性蛋白產(chǎn)品包裝(模擬)
  • 鐵調(diào)素(Hepc)活性蛋白Figure. Gene Sequencing (Extract)
  • APB979Hu61.jpgFigure. SDS-PAGE
  • 鐵調(diào)素(Hepc)活性蛋白Figure. Western Blot
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

Figure. The binding activity of Hepc with FPN.
Hepcidin (Hepc) is a regulator of iron metabolism. Hepcidin inhibits iron transport by binding to the iron export channel ferroportin which is located on the basolateral surface of gut enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepcidin ultimately breaks down the transporter protein in the lysosome. Inhibiting ferroportin prevents iron from being exported and the iron is sequestered in the cells. Besides, Ferroportin (FPN) has been identified as an interactor of Hepc, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Hepc and recombinant human FPN. Briefly, Hepc were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FPN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Hepc pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of Hepc and FPN was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
EPB979Hu61鐵調(diào)素(Hepc)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB979Hu01鐵調(diào)素(Hepc)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB979Hu61鐵調(diào)素(Hepc)活性蛋白Cell?culture;?Activity?Assays.
CPB979Hu12鐵調(diào)素(Hepc)牛血清白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPB979Hu22鐵調(diào)素(Hepc)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPB979Hu23鐵調(diào)素(Hepc)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CPB979Hu21鐵調(diào)素(Hepc)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
PAB979Hu07鐵調(diào)素(Hepc)多克隆抗體IHC
PAB979Hu09鐵調(diào)素(Hepc)多克隆抗體WB; IHC; ICC; IP.
PAB979Hu06鐵調(diào)素(Hepc)多克隆抗體WB; IHC; ICC; IP.
PAB979Hu01鐵調(diào)素(Hepc)多克隆抗體WB; IHC
PAB979Hu08鐵調(diào)素(Hepc)多克隆抗體WB; IHC; ICC; IP.
LAB979Hu81鐵調(diào)素(Hepc)多克隆抗體(異硫氰酸熒光素標(biāo)記)WB; IHC; ICC; IF.
LAB979Hu71鐵調(diào)素(Hepc)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
MAB979Hu26鐵調(diào)素(Hepc)單克隆抗體Testing in progress
MAB979Hu22鐵調(diào)素(Hepc)單克隆抗體WB; IHC; ICC; IP.
MAB979Hu21鐵調(diào)素(Hepc)單克隆抗體IHC
RAB979Hu21鐵調(diào)素(Hepc)重組抗體WB; IF; ICC; IHC; IP; FCM.
SEB979Hu鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
CEB979Hu鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCB979Hu鐵調(diào)素(Hepc)檢測試劑盒(化學(xué)發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
CCB979Hu鐵調(diào)素(Hepc)檢測試劑盒(化學(xué)發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMB979Hu鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSB979Hu11鐵調(diào)素(Hepc)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
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PLOS?ONES-Propargyl-Cysteine, a?Novel?Hydrogen?Sulfide?Donor,?Inhibits?Inflammatory?Hepcidin?and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway.[pubmed:27649298]
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Oxidative Medicine and Cellular LongevityAcidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation[pubmed:29147463]
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The Japanese Society of HematologyHepcidin suppression in β?thalassemia is associated with the down?regulation of atonal homolog 8[pubmed:28405918]
TuberculosisModulation of iron status biomarkers in tuberculosis-diabetes co-morbidity[10.1016/j.tube.2017.11.011]
Oman Medical JournalThe Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.[pubmed:28584599]
Cellular?Physiology and BiochemistryThe Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice[Pubmed:29843136]
Cellular?Microbiology?inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages[Pubmed:29470856]
Journal of Histochemistry & CytochemistryAcute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney[Pubmed:29873589]
Clinical and Experimental?MedicineIncreased NGAL level associated with iron store in chronic kidney disease with anemia[Pubmed:29909502]
HematologyRelationship between obesity and iron deficiency anemia: is there a role of hepcidin?[Pubmed:29318945]
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Food & FunctionEffect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats[Doi: 10.1039/C8FO01696H]
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Naunyn Schmiedebergs Arch Pharmacol.3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitro[Pubmed: 31659405]
DEVELOPMENT GROWTH & DIFFERENTIATIONH2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axis[Pubmed: 32012242]
Frontiers in PhysiologyIncreased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers[Pubmed: 32038278]
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JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGYCirculating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in?…[Pubmed: 33065276]
Sleep BreathRelation of serum hepcidin levels and restless legs syndrome in chronic hemodialysis patients[Pubmed: 33029690]
journal of trace elements in experimental medicineDifference in iron metabolism may partly explain sex-related variability in the manifestation of Wilson's disease[Pubmed: 32937238]
reproduction in domestic animalsExpression of iron regulatory proteins in full term swine placenta[Pubmed: 32449967]
Neurobiology of AgingEstrogen deficiency is associated with brain iron deposition via upregulation of hepcidin expression in aged female mice[Pubmed: 32920472]
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COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION[]
Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection[]
Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с?…[]
Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной …[]
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BiometalsIron metabolism is disturbed and anti-copper treatment improves but does not normalize iron metabolism in Wilson's disease[33555495]
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Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study[]
Reports of Biochemistry & Molecular BiologyEvaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization[]
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J Trace Elem Med Biol Caffeine and alcohol-friends or foes of human iron stores?[Pubmed:35063815]
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Food BioscienceRoles of homopolymeric apoferritin in alleviating alcohol-induced liver injury[]