Histone Deacetylase 6 (HDAC6) is an enzyme that belongs to the histone deacetylase family. It is responsible for removing acetyl groups from lysine residues in histone proteins, thereby playing a crucial role in regulating gene expression through chromatin remodeling. HDAC6 is unique among HDACs in that it is primarily localized in the cytoplasm and is involved in a variety of cellular processes, including protein degradation, cell motility, and autophagy.Besides,ANuclear Receptor Corepressor 1 (NCOR1) has been identified as an interactor of HDAC6, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse HDAC6 and recombinant human NCOR1. Briefly, HDAC6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NCOR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HDAC6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat HDAC6 and recombinant human NCOR1 was shown in Figure 1, the EC50?for this effect is 0.01ug/mL.