同源框蛋白D13(HOXD13)活性蛋白
Active Homeobox Protein D13 (HOXD13)
HOX4I; SPD
- 編號(hào)APP948Hu01
- 物種Homo sapiens (Human,人)相同的名稱(chēng),不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點(diǎn)9.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)
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活性實(shí)驗(yàn)

Homeobox protein Hox - D13 (HOXD13) is a key transcription factor in the homeobox family.HOXD13 is crucial for tissue and organ patterning and differentiation, especially in axial skeleton and limb development. Abnormalities in HOXD13, like mutations or abnormal expressions, can cause developmental disorders such as synpolydactyly, highlighting its importance in normal morphological development.Besides,Hoxd-13?can?bind?to?and?transactivate?the?Shh?promoter?in?vitro, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human HOXD13 and recombinant human SHH .Briefly, biotin-linked HOXD13 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to SHH-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of HOXD13 and SHH was shown in Figure 1, the EC50?for this effect is 0.019ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APP948Hu01 | 同源框蛋白D13(HOXD13)活性蛋白 | Cell?culture;?Activity?Assays. |
RPP948Hu01 | 同源框蛋白D13(HOXD13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |