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白介素17受體C(IL17RC)活性蛋白

Active Interleukin 17 Receptor C (IL17RC)

IL17-RL; IL17R-C; IL17Rhom; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; ZcytoR14

  • 白介素17受體C(IL17RC)活性蛋白產(chǎn)品包裝(模擬)
  • 白介素17受體C(IL17RC)活性蛋白產(chǎn)品包裝(模擬)
  • APD350Hu01.jpgFigure. SDS-PAGE; Sample: Active recombinant IL17RC, Human.
  • 白介素17受體C(IL17RC)活性蛋白Figure. Western Blot; Sample: Recombinant IL17RC, Human.
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

IL17RC (Interleukin-17 receptor C) is a receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. This protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. A binding ELISA assay was conducted to detect the association of IL17RC with IL17RA. Briefly, IL17RC were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RC with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPD350Hu01白介素17受體C(IL17RC)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APD350Hu01白介素17受體C(IL17RC)活性蛋白Cell?culture;?Activity?Assays.
PAD350Hu01白介素17受體C(IL17RC)多克隆抗體WB

參考文獻(xiàn)

雜志參考文獻(xiàn)
Frontiers in PharmacologyTraditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal?…[Pubmed:29375374]