Mechanism: Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance.It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line . To test the bioactivity of LMTK3 ,MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well in serum-free standard DMEM after adding to various concentrations of LMTK3 protein. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly，10 μl of CCK-8 solution was added to each well of the plate，then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C .
Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.