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抑瘤素M(OSM)活性蛋白

Active Oncostatin M (OSM)

OS-M

  • 抑瘤素M(OSM)活性蛋白產(chǎn)品包裝(模擬)
  • 抑瘤素M(OSM)活性蛋白產(chǎn)品包裝(模擬)
  • APA110Hu61.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development. To test the effect of OSM on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human OSM. After incubated for 72 hours,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with OSM for 72 hours observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human OSM for 72 hours. The result was shown in Figure 2. It was obvious that recombinant human OSM significantly increased cell viability of TF-1 cells, the EC50 was 0.08 ng/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
EPA110Hu61抑瘤素M(OSM)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA110Hu01抑瘤素M(OSM)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA110Hu61抑瘤素M(OSM)活性蛋白Cell?culture;?Activity?Assays.
APA110Hu01抑瘤素M(OSM)活性蛋白Cell?culture;?Activity?Assays.
PAA110Hu06抑瘤素M(OSM)多克隆抗體WB,IHC
PAA110Hu01抑瘤素M(OSM)多克隆抗體WB; IHC; ICC; IP.
MAA110Hu22抑瘤素M(OSM)單克隆抗體WB; IHC; ICC; IP.
SEA110Hu抑瘤素M(OSM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA110Hu抑瘤素M(OSM)檢測試劑盒(化學(xué)發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA110Hu抑瘤素M(OSM)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA110Hu01抑瘤素M(OSM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
Journal of Obstetrics and Gynaecology ResearchOncostatin M as a target biological molecule of preeclampsia[PubMed: 20149034]
J NeuroinflammationOncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicity via Smad2/4 in Human Renal Proximal Tubular Epithelial Cells[Pubmed:27287400]
22Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material.[pubmed:28582717]
Advanced?Functional?MaterialsFungal Component Coating Enhances Titanium Implant‐Bone Integration[Doi: 10.1002/adfm.201804483]