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尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白

Active Plasminogen Activator, Urokinase Receptor (uPAR)

CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3

  • 尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白產(chǎn)品包裝(模擬)
  • 尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白產(chǎn)品包裝(模擬)
  • APA141Ra01.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

Plasminogen Activator, Urokinase Receptor (uPAR) is the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. The urokinase receptor (uPAR) was identified as the membrane receptor of the serine protease urokinase (uPA), thereby implicated in the plasminogen activation cascade and regulation of pericellular proteolysis, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat uPAR and recombinant human uPA. Briefly, biotin-linked uPAR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to uPA-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of uPAR and uPA was shown in Figure 1, the EC50?for this effect is 0.223 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。

參考文獻

雜志參考文獻
International Journal of CancerDietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice[Wiley: source]
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism[PubMed: PMC3623607]
Biomarker insightsDecreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)[PubMed: PMC3762604]
Am J Emerg MedClinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic guidance of sepsis[PubMed: 26615223]
Archives of Clinical Infectious DiseasesEvaluation of Diagnostic Value of Soluble Urokinase-Type Plasminogen Activator Receptor in Sepsis[Archcid: 26346]
The American Journal of Emergency Medicine Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic Cavia (Guinea pig )idance of sepsis[science:S0735675715009559]
Journal of Clinical and Experimental HepatologyEvaluation of Plasma Urokinase-Type Plasminogen Activator Receptor (UPAR) in Patients With Chronic Hepatitis B, C and Non-Alcoholic Fatty Liver Disease (NAFLD)?…[10.1016:j.jceh.2018.02.001]
Turkish journal of trauma & emergency surgeryThe diagnostic value of serum urokinase-type plasminogen activator receptor in acute appendicitis[Pubmed: 31475329]
CELL TRANSPLANTATIONPredictive Value of Degranulating Factors of Neutrophils in Massive Cerebral Infarction[33787356]