S100鈣結(jié)合蛋白A7(S100A7)活性蛋白
Active S100 Calcium Binding Protein A7 (S100A7)
S100-A7; PSOR1; S100A7c; Psoriasin 1
- 編號(hào)APC035Mu01
- 物種Mus musculus (Mouse,小鼠)相同的名稱(chēng),不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)5.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格10μg50μg200μg1mg5mg
- 價(jià)格暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)暫無(wú)報(bào)價(jià)
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活性實(shí)驗(yàn)

S100 Calcium Binding Protein A7 (S100A7) is a calcium - binding protein predominantly expressed in epithelial cells. It plays roles in innate immunity, inflammation, and cell growth regulation. Linked to psoriasis and cancer, S100A7 exhibits antibacterial activity.S100A7 interacts with S100A11, forming heterodimers that may enhance their functions in cellular signaling and inflammation - related processes.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse S100A7 and recombinant human S100A11. Briefly, S100A7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A11-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-S100A7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse S100A7 and recombinant human S100A11 was shown in Figure 1, the EC50?for this effect is 0.066ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPC035Mu01 | S100鈣結(jié)合蛋白A7(S100A7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC035Mu02 | S100鈣結(jié)合蛋白A7(S100A7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APC035Mu01 | S100鈣結(jié)合蛋白A7(S100A7)活性蛋白 | Cell?culture;?Activity?Assays. |
PAC035Mu02 | S100鈣結(jié)合蛋白A7(S100A7)多克隆抗體 | WB; IHC; ICC; IP. |
PAC035Mu01 | S100鈣結(jié)合蛋白A7(S100A7)多克隆抗體 | WB |
LAC035Mu71 | S100鈣結(jié)合蛋白A7(S100A7)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEC035Mu | S100鈣結(jié)合蛋白A7(S100A7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC035Mu | S100鈣結(jié)合蛋白A7(S100A7)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Archives of Dermatological Research | Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters[SpringerLink: t07u24t43r357l15] |
J Proteome Res | Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach[PubMed: 25807139] |
Exp Dermatol | S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signaling[Pubmed:27060579] |
Experimental Dermatology | S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signalling[pubmed:27060579] |
Experiental And Therapeutic Medicine | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription[pubmed:28672924] |
Scientific Reports | The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function.[pubmed:29142248] |
Journal of the European Academy of Dermatology and Venereology | A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosis[Pubmed:28681537] |
Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique[Pubmed:29374283] |
Neuroscience?Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state[Pubmed:30019218] |
Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection[Pubmed: 31706945] |
Mediators of Inflammation | Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris[] |