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絲氨酸蛋白酶抑制劑A10(SERPINA10)活性蛋白

Active Serpin A10 (SERPINA10)

PZI; ZPI; Serine(Cysteine)Proteinase Inhibitor,Clade A(Alpha-1 Antiproteinase,Antitrypsin)Member 10; Protein Z-Related Protease Inhibitor; Protein Z Dependent Protease Inhibitor

  • 絲氨酸蛋白酶抑制劑A10(SERPINA10)活性蛋白產(chǎn)品包裝(模擬)
  • 絲氨酸蛋白酶抑制劑A10(SERPINA10)活性蛋白產(chǎn)品包裝(模擬)
  • APC010Hu01.jpgFigure. SDS-PAGE
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor?X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor?X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor?X at 37 ℃ for 30?minutes. Loading 50?μL 20?μM substrate to start the reaction including a substrate blank containing 50?μL of assay buffer and 50?μL of 20?μM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor?X activity. The inhibition IC50 was <75 nM.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

參考文獻(xiàn)

雜志參考文獻(xiàn)
Thrombosis ResearchPlasma concentration of protein Z and protein Z-dependent protease inhibitor in patients with haemophilia A[PubMed: 23269381]
Clin Appl Thromb HemostPlasma Concentrations of Protein Z and Protein Z-Dependent Protease Inhibitor in Patients With Essential Thrombocythemia[PubMed: 25783867]
AngiologyAssessment of the Procoagulant Activity of Microparticles and the Protein Z System in Patients Undergoing Off-Pump Coronary Artery Bypass Surgery.[pubmed:28464697]