抗微管蛋白β(TUBb)單克隆抗體
Anti-Tubulin Beta (TUBb) Monoclonal Antibody
TUB-B; TUBB5; TUBB1; M40
- 編號CAB870Mi22
- 物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.65mg/mL
- 且適物種Hamster (Chinese hamster，倉鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
- 應(yīng)用Loading Control of WB
- 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 716¥ 1672¥ 2388¥ 5970¥ 23880
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 10μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: Human MCF7 cell;
Primary Ab: 40μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
AF488 staining on IF;
Sample: HepG2 cell
TRITC Phalloidin at 100 nM (shown in pseudo color red)
Primary Ab: 20μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
Figure. Western Blot Sample: Recombinant TUBb
Western Blot analysis of TUBb in various cell lines
Lane1: HL60 whole cell lysate
Lane2: THP-1 whole cell lysate
Lane3: MDA-MB-435S whole cell lysate
Lane4: T-47D whole cell lysate
Lysates/proteins at 20μg per lane.
Primary Ab: anti-TUBb antibody (CAB870Mi22) at 0.3ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 50KD
Observed MW: 50KD
Exposure time: 1 min
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Canine Kidney lysate; Lane4: Gallus Cerebrum lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Cerebrum lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Cavia Kidney lysate; Lane10: Caprine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: HL60 cell lysate; Lane3: THP1 cell lysate; Lane4: OS-RC-2 cell lysate; Lane5: HEC-1B cell lysate; Lane6: MDA-MB-435S cell lysate; Lane7: T-47D cell lysate
Primary Ab: 0.5μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Heart lysate; Lane3: Mouse Cerebellum lysate; Lane4: Porcine Cerebrum lysate; Lane5: Bovine Kidney lysate; Lane6: Rabbit Pancreas lysate
Primary Ab: 0.0003μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Samples: Lane1: SH-SY5Y cell lysate; Lane2: Hela cell lysate; Lane3: HepG2 cell lysate; Lane4: PC3 cell lysate;
Primary Ab: 0.1μg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對TUBb的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別TUBb。
用法
Western blotting: 0.0003-0.1ug/ml
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CAB870Mi01 | 抗微管蛋白β(TUBb)多克隆抗體 | Loading Control of WB. |
| CAB870Mi22 | 抗微管蛋白β(TUBb)單克隆抗體 | Loading Control of WB |
| SEB870Mi | 微管蛋白β(TUBb)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB870Mi | 微管蛋白β(TUBb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| archives of pharmacal research | Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues[pubmed:27747473] |
| archiv der pharmazie (Weinheim). | New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition.[pubmed:28150327] |
| ACS?Applied?Materials?&?Interfaces | Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture[pubmed:28112513] |
| Future?Medicinal Chemistry | Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents[Pubmed:29779400] |
| Future?Medicinal Chemistry | Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization[Pubmed:29966433] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids[Pubmed: 30237167] |
| Journal?of?Enzyme?Inhibition?and?Medicinal?Chemistry | Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors[Pubmed: 30191744] |
| European Journal of Medicinal Chemistry | Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study[Pubmed: 31536891] |
| ACS Sensors | Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform[Pubmed: 32207606] |
| Bioorganic Chemistry | Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their?…[Pubmed: 32325332] |
| BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats[Pubmed: 33028437] |
| European Journal of Medicinal Chemistry | Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon?…[Pubmed: 33158579] |
| J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells[33300466] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells[34837026] |