果糖二磷酸醛縮酶A(ALDOA)多克隆抗體
Polyclonal Antibody to Aldolase A, Fructose Bisphosphate (ALDOA)
FBA; ALDO-A; Fructose 1,6 Bisphosphate Aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase
- 編號(hào)PAA642Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPA642Hu01-果糖二磷酸醛縮酶A(ALDOA)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 565¥ 1319¥ 1884¥ 4710¥ 18840
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Rat Colon Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human ALDOA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ALDOA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Skeletal muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ALDOA Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: HepG2 cell lysate; Primary Ab: 2μg/ml Rabbit Anti-Human ALDOA Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: Mouse Heart lysate; <br/>Primary Ab: 2μg/mL Rabbit Anti-Human ALDOA Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Human Liver lysate; <br/>Primary Ab: 2μg/ml Rabbit Anti-Human ALDOA Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Liver lysate; Lane3: Rat Cerebrum lysate; Lane4: Rat Small intestine lysate; Lane5: Rat Skeletal muscle lysate<br/>Primary Ab: 0.2μg/ml Rabbit Anti-Human ALDOA Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human ALDOA Polyclonal Antibody (Catalog # PAA642Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)ALDOA的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別ALDOA。
用法
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA642Hu01 | 果糖二磷酸醛縮酶A(ALDOA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA642Hu01 | 果糖二磷酸醛縮酶A(ALDOA)多克隆抗體 | WB; IHC; ICC; IP. |
| FAA642Hu01 | 抗果糖二磷酸醛縮酶A(ALDOA)多克隆抗體 | Flow cytometry. |
| MAA642Hu21 | 果糖二磷酸醛縮酶A(ALDOA)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA642Hu | 果糖二磷酸醛縮酶A(ALDOA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA642Hu | 果糖二磷酸醛縮酶A(ALDOA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |