彈性蛋白(ELN)多克隆抗體
Polyclonal Antibody to Elastin (ELN)
WBS, WS, SVAS; Tropoelastin; Supravalvular Aortic Stenosis; Williams-Beuren Syndrome
- 編號PAB337Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB337Hu01-彈性蛋白(ELN)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.42mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 598¥ 1394¥ 1992¥ 4980¥ 19920
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colon Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Sample: Human Spleen Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Figure. Western Blot; Sample: Recombinant ELN, Human.
Western Blot; Sample: Human Lung lysate
Primary Ab: 1μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Skin lysate
Primary Ab: 2μg/ml Rabbit Anti-Human ELN Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ELN的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別ELN。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB337Hu01 | 彈性蛋白(ELN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB337Hu61 | 彈性蛋白(ELN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB337Hu21 | 彈性蛋白(ELN)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPB337Hu31 | 彈性蛋白(ELN)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAB337Hu01 | 彈性蛋白(ELN)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB337Hu08 | 彈性蛋白(ELN)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB337Hu81 | 彈性蛋白(ELN)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB337Hu71 | 彈性蛋白(ELN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB337Hu21 | 彈性蛋白(ELN)單克隆抗體 | WB |
| MAB337Hu22 | 彈性蛋白(ELN)單克隆抗體 | WB |
| MAB337Hu29 | 彈性蛋白(ELN)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB337Hu24 | 彈性蛋白(ELN)單克隆抗體 | WB |
| MAB337Hu23 | 彈性蛋白(ELN)單克隆抗體 | WB; IHC |
| MAB337Hu25 | 彈性蛋白(ELN)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB337Hu27 | 彈性蛋白(ELN)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB337Hu02 | 抗彈性蛋白(ELN)單克隆抗體 | FCM |
| HEB337Hu | 彈性蛋白(ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB337Hu | 彈性蛋白(ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB337Hu | 抗彈性蛋白抗體(Anti-ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCB337Hu | 彈性蛋白(ELN)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| KSB337Hu01 | 彈性蛋白(ELN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinical & Experimental Immunology | Induction of elastin expression in vascular endothelial cells relates to hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum anti-endothelial cell antibodies[PubMed: 22288597] |
| Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.[Pubmed: 24739658] |
| Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins[Pubmed:25732262] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592] |
| Nutrition & Diabetes | Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling.[pubmed:27941938] |
| FREE RADICAL BIOLOGY AND MEDICINE | Anti-ageing effects of Ubiquinone and Ubiquinol in a senescence model of human dermal fibroblasts[33482334] |
| GENE THERAPY DNA VECTOR VTvaf17, METHOD OF PRODUCTION; ESCHERICHIA COLI STRAIN SCS110-AF, METHOD OF PRODUCTION; ESCHERICHIA??-[] | |
| ARCHIV?EUROMEDICA | CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE[] |