非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體
Polyclonal Antibody to Enolase 1 (ENO1)
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
- 編號PAB449Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB449Mu01-非神經(jīng)元性烯醇化酶(ENO1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 616¥ 1436¥ 2052¥ 5130¥ 20520
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NNE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;<br/>Sample: MCF7 cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Mouse ENO1 Antibody<br/>Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
Figure. Western Blot; Sample: Recombinant ENO1, Mouse.
Knockout Varification: <br/>Lane 1: Wild-type MCF7 cell lysate; <br/>Lane 2: NNE knockout MCF7 cell lysate; <br/>Predicted MW: 36,47kDa <br/>Observed MW: 50kDa<br/>Primary Ab: 1μg/ml Rabbit Anti-Mouse NNE Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 2μg/mL Rabbit Anti-Mouse NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Mammary gland lysate;
Primary Ab: 2μg/mL Rabbit Anti-Mouse NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 2μg/mL Rabbit Anti-Mouse NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Colon lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse NNE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Heart lysate; Lane3: Porcine Liver lysate;
Primary Ab: 0.005μg/ml Rabbit Anti-Mouse ENO1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Porcine Skeletal muscle lysate; Lane2: LO2 cell lysate; Lane3: PC3 cell lysate;
Primary Ab: 0.005μg/ml Rabbit Anti-Mouse ENO1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ENO1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別ENO1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB449Mu01 | 非神經(jīng)元性烯醇化酶(ENO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Mu01 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體 | WB,IHC,ICC/IF |
| LAB449Mu71 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體(生物素標(biāo)記) | WB |
| LAB449Mu81 | 非神經(jīng)元性烯醇化酶(ENO1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| SEB449Mu | 非神經(jīng)元性烯醇化酶(ENO1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB449Mu | 非神經(jīng)元性烯醇化酶(ENO1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB449Mu01 | 非神經(jīng)元性烯醇化酶(ENO1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor[PubMed: 20557064] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules[Pubmed:27172385] |
| Journal of?Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia[Pubmed:29654921] |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis[Pubmed: 33062681] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212] |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma[Pubmed:35610567] |