心型脂肪酸結(jié)合蛋白(H-FABP)多克隆抗體
Polyclonal Antibody to Heart-type Fatty Acid Binding Protein (H-FABP)
MDGI; FABP11; H-FABP; O-FABP; Hfabp; FABP3; Fatty Acid Binding Protein 3, Muscle And Heart; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-ty
- 編號PAB243Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB243Hu01-心型脂肪酸結(jié)合蛋白(H-FABP)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.41mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 598¥ 1394¥ 1992¥ 4980¥ 19920
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human FABP3 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human FABP3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Skeletal muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human FABP3 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human H-FABP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Skeletal muscle Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human H-FABP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Rat Testis Tissue; <br/>Primary Ab: 20ug/ml Rabbit Anti-Human H-FABP Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20ug/ml Rabbit Anti-Human H-FABP Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
Figure. Western Blot; Sample: Recombinant FABP3, Human.
Western Blot; Sample: Porcine Heart lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human FABP3 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Rat Heart lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human FABP3 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Mouse Heart lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human FABP3 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Rat Heart lysate; Lane4: Rat Kidney lysate<br/>Primary Ab: 0.1μg/ml Rabbit Anti-Human H-FABP Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
Human MDA-MB-231 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human H-FABP Polyclonal Antibody (Catalog PAB243Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對H-FABP的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別H-FABP。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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參考文獻
| 雜志 | 參考文獻 |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion[Pubmed:29393473] |
| Oncology?Letters | Hydrogen?rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus?activated kinase 2/signal transducer and activator of?…[Pubmed:29928398] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure[Pubmed: 30230922] |