70kDa熱休克蛋白1A(HSPA1A)多克隆抗體
Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- 編號(hào)PAB081Bo01
- 物種Bos taurus; Bovine (Cattle，牛)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB081Bo01-70kDa熱休克蛋白1A(HSPA1A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 720¥ 1680¥ 2400¥ 6000¥ 24000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Bovine Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Bovine HSPA1A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Bovine Testis Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Testis Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Bovine Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant HSPA1A, Bovine.
Western Blot; Sample: Bovine Cerebrum lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Bovine Kidney lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Bovine Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Bovine Cerebrum lysate; Lane2: Bovine Liver lysate; Lane3: Bovine Kidney lysate; Lane4: HepG2 cell lysate; Lane5: PC3 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Bovine HSPA1A Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)HSPA1A的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HSPA1A。
用法
Western blottin:g: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB081Bo01 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB081Bo01 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB; IHC |
| LAB081Bo81 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB081Bo71 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體(生物素標(biāo)記) | WB |
| MAB081Bo21 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB081Bo | 70kDa熱休克蛋白1A(HSPA1A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB081Bo | 70kDa熱休克蛋白1A(HSPA1A)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB081Bo01 | 70kDa熱休克蛋白1A(HSPA1A)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Neuroinflammation | Identification of potential biomarkers of gold nanoparticle toxicity in rat brains[PubMed: 22691312] |
| Mediators Inflamm.? | Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs[Pubmed:Pmc4324980] |
| Eur J Pharmacol. | Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats[Pubmed:24508522] |
| Pharmacol Rep. | Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice[Pubmed:25443743] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics[pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?…[Pubmed:29379533] |
| metabolic brain disease | Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus[Pubmed: 32172517] |
| EXPERIMENTAL CELL RESEARCH | Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice[Pubmed: 32439493] |