音猬因子(SHH)多克隆抗體
Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)
HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N
- 編號PAB831Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPB831Hu01-音猬因子(SHH)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 598¥ 1394¥ 1992¥ 4980¥ 19920
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human SHH Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Western Blot; Sample: PANC1 cell lysate Primary Ab: 0.5μg/ml Rabbit Anti-Human SHH Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Kidney lysate; Lane3: Rat Liver lysate; Lane4: Rat Kidney lysate;
Primary Ab: 0.5μg/ml Rabbit Anti-Human SHH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對SHH的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SHH。
用法
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB831Hu01 | 音猬因子(SHH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB831Hu61 | 音猬因子(SHH)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB831Hu01 | 音猬因子(SHH)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB831Hu61 | 音猬因子(SHH)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB831Hu01 | 音猬因子(SHH)多克隆抗體 | WB; IHC; ICC/IF |
| MAB831Hu22 | 音猬因子(SHH)單克隆抗體 | WB; IHC |
| MAB831Hu21 | 音猬因子(SHH)單克隆抗體 | WB; IHC |
| MAB831Hu24 | 音猬因子(SHH)單克隆抗體 | WB |
| RAB831Hu21 | 音猬因子(SHH)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB831Hu23 | 音猬因子(SHH)單克隆抗體 | IHC |
| SEB831Hu | 音猬因子(SHH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB831Hu | 音猬因子(SHH)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB831Hu | 音猬因子(SHH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer[y2016:0077095.html] |
| Annals of the Rheumatic Diseases | Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis[pubmed:28495672] |