肝癌源性生長因子(HDGF)多克隆抗體
Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)
HMG1L2; High-Mobility Group Protein 1-Like 2
- 編號PAA624Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPA624Mu01-肝癌源性生長因子(HDGF)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.38mg/ml
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 583¥ 1361¥ 1944¥ 4860¥ 19440
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Mouse Uterus Tissue; Primary Ab: 30μg/ml Rabbit Anti-Mouse HDGF Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Heart Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Spleen Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Mouse HDGF Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
Figure. Western Blot; Sample: Recombinant HDGF, Mouse.
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Mouse Testis lysate; Lane4: Jurkat cell lysate; Lane5: 293T cell lysate;
Primary Ab: 0.2μg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對HDGF的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別HDGF。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA624Mu01 | 肝癌源性生長因子(HDGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA624Mu02 | 肝癌源性生長因子(HDGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA624Mu01 | 肝癌源性生長因子(HDGF)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA624Mu | 肝癌源性生長因子(HDGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA624Mu | 肝癌源性生長因子(HDGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| European Journal of Pharmacology | Hepatoma-derived growth factor promotes the resistance to anti-tumor effects of nordihydroguaiaretic acid in colorectal cancer cells[ScienceDirect: S0014299910007302] |
| J Clin Lab Anal | Association of serum hepatoma‐derived growth factor levels with disease activity in rheumatoid arthritis: A pilot study[Pubmed:35500218] |