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白介素1受體Ⅰ(IL1R1)多克隆抗體

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant IL1R1, Human.
Western Blot; Sample: Raji cell lysate
Primary Ab: 3μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Raji cell lysate;
Lane 2: IL1R1 knockout Raji cell lysate;
Predicted MW: 65kd
Observed MW: 65kd
Primary Ab: 3μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human IL1R1 Polyclonal Antibody (Catalog PAA066Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

該抗體是針對IL1R1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗中能識別IL1R1。

Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

雜志	參考文獻(xiàn)
Clinical & Experimental Immunology	In vivo extravasation induces expression of IL-1R1 in human neutrophils[Wiley: source]
PLos One	The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective Endocarditis[Plosone: 0031490]
Journal of Clinical Apheresis	Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel disease[PubMed: 22267087]
PLoS ONE	A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source]
中國當(dāng)代兒科雜志	IL1R1 基因多態(tài)性與兒童哮喘的相關(guān)性[article:13877]
STEM CELLS AND DEVELOPMENT	IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways[Pubmed: 33107399]
Journal of Integrative Medicine	Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats[Pubmed:35643766]

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA066Hu03	白介素1受體Ⅰ(IL1R1)重組蛋白	8.35
RPA066Hu02	白介素1受體Ⅰ(IL1R1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA066Hu01	白介素1受體Ⅰ(IL1R1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA066Hu01	白介素1受體Ⅰ(IL1R1)活性蛋白	Cell?culture;?Activity?Assays.
PAA066Hu02	白介素1受體Ⅰ(IL1R1)多克隆抗體	WB; IHC; ICC; IP.
PAA066Hu03	白介素1受體Ⅰ(IL1R1)多克隆抗體	WB; ICC/IF
PAA066Hu01	白介素1受體Ⅰ(IL1R1)多克隆抗體	WB,IHC; FCM
LAA066Hu71	白介素1受體Ⅰ(IL1R1)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAA066Hu22	白介素1受體Ⅰ(IL1R1)單克隆抗體	WB; IHC; ICC; IP.
SEA066Hu	白介素1受體Ⅰ(IL1R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA066Hu	白介素1受體Ⅰ(IL1R1)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.