親核素α2(KPNa2)多克隆抗體
Polyclonal Antibody to Karyopherin Alpha 2 (KPNa2)
IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha
- 編號PAE748Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPE748Hu01-親核素α2(KPNa2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 630¥ 1470¥ 2100¥ 5250¥ 21000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human KPNa2 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;<br/>Sample: HepG2 cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Human KPNa2 Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
Figure. Western Blot; Sample: Recombinant KPNa2, Human.
Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Raji cell lysate; Lane4: U2OS cell lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification: <br/>Lane 1: Wild-type HepG2 cell lysate; <br/>Lane 2: KPNa2 knockout HepG2 cell lysate; <br/>Predicted MW: 58kDa <br/>Observed MW: 58kDa<br/>Primary Ab: 3μg/ml Rabbit Anti-Human KPNa2 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human KPNa2 Polyclonal Antibody (Catalog PAE748Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對KPNa2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別KPNa2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPE748Hu01 | 親核素α2(KPNa2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE748Hu01 | 親核素α2(KPNa2)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| MAE748Hu21 | 親核素α2(KPNa2)單克隆抗體 | WB; ICC/IF;FCM |
| MAE748Hu22 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC |
| MAE748Hu23 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC |
| MAE748Hu24 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC |
| MAE748Hu25 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC |
| MAE748Hu26 | 親核素α2(KPNa2)單克隆抗體 | ICC/IF |
| FAE748Hu08 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| FAE748Hu06 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| FAE748Hu04 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| FAE748Hu02 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| SEE748Hu | 親核素α2(KPNa2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME748Hu | 親核素α2(KPNa2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSE748Hu01 | 親核素α2(KPNa2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Oncol Rep.? | KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferation[Pubmed:25109899] |
| Tumor Biology | KPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosis[pubmed:28651492] |
| Head?&?Neck | Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancer[Pubmed:29542209] |
| International Journal of Cancer | Nuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers[34591985] |