線粒體超氧化物歧化酶(SOD2)多克隆抗體
Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
IPO-B; MNSOD; Mn-SOD
- 編號PAB083Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPB083Ra01-線粒體超氧化物歧化酶(SOD2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 652¥ 1520¥ 2172¥ 5430¥ 21720
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Rat Uterus Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Rat Testis Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Rat Kidney Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Rat Skeletal muscle Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Rat Cardiac Muscle Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Rat Liver Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Rat Uterus Tissue<br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Rat Testis Tissue<br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Rat Kidney Tissue<br/>Primary Ab: 20μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
FITC staining on IF;Sample: MCF7 cellPrimary Ab: 20μg/ml Rabbit Anti-Rat SOD2 AntibodySecond Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)
Western Blot; Samples: Lane1: Rat Heart lysate; Lane2: Rat Kidney lysate; Primary Ab: 0.1μg/ml Rabbit Anti-Rat SOD2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Knockout Varification: <br/>Lane 1: Wild-type Hela cell lysate; <br/>Lane 2: SOD2 knockout Hela cell lysate; <br/>Predicted MW: 25kDa <br/>Observed MW: 22kDa<br/>Primary Ab: 1μg/ml Rabbit Anti-Rat SOD2 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Lung lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Liver lysate; Lane3: Porcine Cerebrum lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Porcine Liver lysate; Lane2: SH-SY5Y cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Rat SOD2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對SOD2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SOD2。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB083Ra01 | 線粒體超氧化物歧化酶(SOD2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB083Ra01 | 線粒體超氧化物歧化酶(SOD2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB083Ra01 | 線粒體超氧化物歧化酶(SOD2)多克隆抗體 | WB,IHC,ICC/IF |
| LAB083Ra71 | 線粒體超氧化物歧化酶(SOD2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAB083Ra81 | 線粒體超氧化物歧化酶(SOD2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAB083Ra21 | 線粒體超氧化物歧化酶(SOD2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB083Ra | 線粒體超氧化物歧化酶(SOD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB083Ra | 線粒體超氧化物歧化酶(SOD2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB083Ra01 | 線粒體超氧化物歧化酶(SOD2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Oral Disease | Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats[Pubmed: 24106991] |
| AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure[Pubmed:24443098] |
| Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer[Pubmed:25550558] |
| Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes[PubMed: 26143097] |
| J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva[PubMed: 26608886] |
| Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.[Pubmed:26806551] |
| Basic?Research?in Cardiology | Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and?…[Pubmed:29892894] |
| Journal of Pharmacological?Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome[Pubmed: 30342783] |
| Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance[Pubmed: 31146169] |
| PHARMACOLOGICAL RESEARCH | A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling[Pubmed: 32135248] |
| Biomolecules | Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development[Pubmed: 32751983] |
| Nutrients | Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and??-[34064937] |
| Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients[34832849] |
| Biochimica et Biophysica Acta-Molecular Basis of Disease | α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet[Pubmed:35569738] |