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分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體

Polyclonal Antibody to Secreted Frizzled Related Protein 5 (SFRP5)

SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b

  • 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體產(chǎn)品包裝(模擬)
  • 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體產(chǎn)品包裝(模擬)
  • 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體IHC圖
  • PAC842Ra01.jpgFigure. Western Blot; Sample: Recombinant SFRP5, Rat.
  • 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體Western Blot; Sample: Mouse Pancreas lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Rat SFRP5 Antibody
    Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

該抗體是針對SFRP5的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SFRP5。

用法

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

相關(guān)產(chǎn)品

編號適用物種：Rattus norvegicus (Rat，大鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPC842Ra01分泌型卷曲相關(guān)蛋白5(SFRP5)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC842Ra01分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體WB
LAC842Ra71分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體(生物素標記)WB; IHC; ICC.
MAC842Ra21分泌型卷曲相關(guān)蛋白5(SFRP5)單克隆抗體WB; IHC; ICC; IP.
SEC842Ra分泌型卷曲相關(guān)蛋白5(SFRP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC842Ra分泌型卷曲相關(guān)蛋白5(SFRP5)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
Diabetes Research and Clinical PracticePlasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance[PubMed: 23290274]
European Journal of Clinical InvestigationSfrp5 correlates with insulin resistance and oxidative stress[PubMed: 23398169]
Pediatric ResearchBalanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children[Pubmed: 24603290]
Clin Endocrinol (Oxf).SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children[Pubmed: 24330025]
The Journal of Clinical Endocrinology & MetabolismCirculating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans[Pubmed: 23185036]
AtherosclerosisLow serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease[Pubmed: 24530778]
PLoS One.Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation[Pubmed:Pmc4072672]
J Clin Endocrinol Metab.Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation[Pubmed:24840810]
Oncology LettersSerum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma[Pubmed:25120720]
Diabetes Metab Res Rev.Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls[Pubmed:25139699]
Clinical & Experimental ImmunologyThe wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis[Pubmed:25382802]
Diabetes Metab Res RevCirculating SFRP5 levels are elevated in drug-na[PubMed: 25139699]
J Matern Fetal Neonatal MedPrediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels[PubMed: 26100762]
Eur J Clin InvestSfrp5 associates with beta-cell function in humans[Pubmed:27019073]
Osteoporosis InternationalBone marrow sFRP5 level is negatively associated with bone formation markers[pubmed:27986984]
Diabetes/Metabolism Research and ReviewsPlasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults[pubmed:28303637]
Cardiovascular DiabetologyInverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study[pubmed:28851362]
Arteriosclerosis, Thrombosis, and?Vascular Biology?Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?…[Pubmed:29674475]
ObesityLongitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans[Pubmed:29427376]
J Bas Res Med SciDecreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats[article-1-352-en.pdf]
Molecular?Biology?ReportsThe effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome[Pubmed: 30194556]
Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling[]
British?Journal Of NutritionA diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile[Pubmed: 30375290]
J Clin Endocrinol MetabRole of wnt5a in Metabolic Inflammation in Humans[Pubmed: 30137542]
Frontiers in EndocrinologyAssociation of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients[Pubmed: 32300333]
CLINICAL BIOCHEMISTRYSfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease[Pubmed: 33188773]
Sao Paulo Medical JournalAssociation of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …[Pubmed: 32491086]
Kidney Blood Press ResSecreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway[34469882]
CytokineAssociation of serum uromodulin with adipokines in dependence of type 2 diabetes[34920231]
PLoS OneAssociation of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study[Pubmed:34990470]
JMIR Res ProtocInvestigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition?…[Pubmed:35475790]
DiabetologyEarly Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop?…[]