醛固酮(ALD)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Aldosterone (ALD)
- 編號CCA911Ge
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍7.81-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于2.93pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 4011¥ 5730¥ 25785¥ 48705¥ 401100
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測醛固酮(ALD)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的醛固酮(ALD)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-102 | 99 |
| EDTA plasma(n=5) | 92-99 | 95 |
| heparin plasma(n=5) | 91-103 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的醛固酮(ALD)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中醛固酮(ALD)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-101% | 87-96% | 97-105% | 86-93% |
| EDTA plasma(n=5) | 88-99% | 92-105% | 86-94% | 78-101% |
| heparin plasma(n=5) | 90-101% | 81-105% | 96-105% | 81-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將醛固酮(ALD)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原（標準品或樣本），待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的醛固酮(ALD)呈負相關(guān)。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| MAA911Ge21 | 醛固酮(ALD)單克隆抗體 | ELISA |
| MAA911Ge22 | 醛固酮(ALD)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MEA911Ge | 醛固酮(ALD)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA911Ge | 醛固酮(ALD)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA911Ge | 醛固酮(ALD)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA911Ge | 醛固酮(ALD)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One | High Salt Intake Down-Regulates Colonic Mineralocorticoid Receptors, Epithelial Sodium Channels and 11β-Hydroxysteroid Dehydrogenase Type 2[PlosOne: Source] |
| Physiological Reports | Evidence for glucocorticoid&-mediated hypertension after uninephrectomy[Pubmed: 24303173] |
| International Journal of Endocrinology | Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach[Hindawi: 384583] |
| Int J Endocrinol. | Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach.[Pubmed:24672546] |
| Archives of Physiology and Biochemistry | Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder[Pubmed:26934364] |
| Pflügers Archiv - European Journal of Physiology | H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.[pubmed:27562425] |
| Journal?of?Cardiovascular?Disease?Research | Magnitude of Resistant Hypertension and Impact of Aldosterone to Renin Ratio In Resistant Hypertension[publication:311777221] |
| Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling[pubmed:28187460] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy[pubmed:29240788] |
| Endocrinology | Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy.[pubmed:29059290] |
| Journal of?Thoracic?and Cardiovascular?Surgery | Acute adrenal cortex injury during cardiopulmonary bypass in a canine model[Pubmed:29753511] |
| American Journal of Physiology-renal Physiology | ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid load[Pubmed: 31188029] |
| Cells | MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model[Pubmed: 31540304] |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats[Pubmed: 32349626] |
| European Journal of Pharmacolog | Preventive preclinical efficacy of intravenously administered sphingosine-1-phosphate (S1P) in strengthening hypoxia adaptive responses to acute and sub-chronic?…[] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice[33303990] |
| Pflugers Arch | Decreased KLHL3 expression is involved in the activation of WNK-OSR1/SPAK-NCC cascade in type 1 diabetic mice[33432425] |