白介素33(IL33)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Interleukin 33 (IL33)
DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules
- 編號SCB980Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍1.37-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于0.51pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3629¥ 5184¥ 23328¥ 44064¥ 362880
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測白介素33(IL33)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素33(IL33)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-96 | 83 |
| EDTA plasma(n=5) | 81-95 | 90 |
| heparin plasma(n=5) | 98-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的白介素33(IL33)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素33(IL33)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-96% | 83-97% | 91-98% | 80-105% |
| EDTA plasma(n=5) | 98-105% | 92-101% | 80-104% | 95-105% |
| heparin plasma(n=5) | 92-101% | 94-105% | 89-103% | 78-91% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將白介素33(IL33)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的白介素33(IL33)與連接于固相載體上的抗體結合，然后加入生物素化的白介素33(IL33)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發(fā)射光強度和樣品中的白介素33(IL33)呈正相關。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB980Mu02 | 白介素33(IL33)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB980Mu01 | 白介素33(IL33)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB980Mu01 | 白介素33(IL33)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB980Mu02 | 白介素33(IL33)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB980Mu81 | 白介素33(IL33)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB980Mu71 | 白介素33(IL33)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MEB980Mu | 白介素33(IL33)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB980Mu | 白介素33(IL33)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB980Mu | 白介素33(IL33)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB980Mu | 白介素33(IL33)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Acta Histochem | CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice[PubMed: 26456520] |
| Journal of Interferon & Cytokine Research | Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis[Pubmed:26540312] |
| Journal of Interferon & Cytokine Research | The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.[pubmed:27348082] |
| Archives of Dermatological Research | IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo[pubmed:27388717] |
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| Cell Reports | Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s[Pubmed: 32101749] |
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| Traditional Medicine Research | Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …[] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota[] | |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus[34395633] |
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| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling[] |