粘蛋白5AC(MUC5AC)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- 編號SCA756Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍13.7-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.1pg/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3528¥ 5040¥ 22680¥ 42840¥ 352800
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產品包裝(模擬)
產品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測粘蛋白5AC(MUC5AC)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將粘蛋白5AC(MUC5AC)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的粘蛋白5AC(MUC5AC)與連接于固相載體上的抗體結合，然后加入生物素化的粘蛋白5AC(MUC5AC)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發(fā)射光強度和樣品中的粘蛋白5AC(MUC5AC)呈正相關。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA756Hu01 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA756Hu01 | 粘蛋白5AC(MUC5AC)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA756Hu81 | 粘蛋白5AC(MUC5AC)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA756Hu71 | 粘蛋白5AC(MUC5AC)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA756Hu21 | 粘蛋白5AC(MUC5AC)單克隆抗體 | WB |
| MAA756Hu22 | 粘蛋白5AC(MUC5AC)單克隆抗體 | WB |
| LAA756Hu72 | 粘蛋白5AC(MUC5AC)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA756Hu | 粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA756Hu | 粘蛋白5AC(MUC5AC)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA756Hu | 粘蛋白5AC(MUC5AC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA756Hu01 | 粘蛋白5AC(MUC5AC)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA756Hu01 | 粘蛋白5AC(MUC5AC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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