防御素β1(DEFb1)重組蛋白
Recombinant Defensin Beta 1 (DEFb1)
B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1
- 編號(hào)RPB373Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞膜, 分泌
- 預(yù)測(cè)分子量8.9kDa
- 實(shí)際分子量10kDa(差異分析請(qǐng)參閱說明書)
- 片段與標(biāo)簽Val22~Ser69 with N-terminal His Tag
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 80%
- 等電點(diǎn)7.7
- 應(yīng)用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB373Mu02 | 防御素β1(DEFb1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB373Mu01 | 防御素β1(DEFb1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB373Mu01 | 防御素β1(DEFb1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB373Mu02 | 防御素β1(DEFb1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB373Mu71 | 防御素β1(DEFb1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB373Mu | 防御素β1(DEFb1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB373Mu | 防御素β1(DEFb1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line[Asm: Source] |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese[Pubmed: 24124699] |
| International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula[Doi: 10.1111] |
| Molecular Nutrition Food Research | Green?tea?polyphenol?epigallocatechin-3-gallate?improves?epithelial?barrier?function?by?inducing?the?productionof?antimicrobial?peptide?pBD-1?and?pBD-2?in?monolayers?of?porcine?intestinal?epithelial?IPEC-J2?cells.[Pubmed:26991948] |
| Frontiers in Microbiology | Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense[Pubmed:29997590] |
| International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase?…[Pubmed: 30342347] |
| cyberleninka | EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS[] |
| Fundamental & clinical pharmacology | Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo[Pubmed: 31313350] |
| Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis[Pubmed: 31927460] |
| Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport[33375218] |
| Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus[34362937] |
| J Mol Histol | Isolation, culture, and identification of ceruminous gland cells[Pubmed:35113280] |