防御素β2(DEFb2)重組蛋白
Recombinant Defensin Beta 2 (DEFb2)
B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
- 編號RPA072Po01
- 物種Sus scrofa; Porcine (Pig，豬)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量35.0kDa
- 實際分子量35kDa(差異分析請參閱說明書)
- 片段與標簽Ile22~Arg69 with N-terminal His and GST Tag
- 緩沖液成份100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點8.9
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 984¥ 2460¥ 4920¥ 14760¥ 36900
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產品包裝(模擬)
產品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA072Po01 | 防御素β2(DEFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA072Po01 | 防御素β2(DEFb2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA072Po21 | 防御素β2(DEFb2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA072Po | 防御素β2(DEFb2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA072Po | 防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Livestock Science | The effect of Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets[ScienceDirect: S1871141311000126] |
| Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line[Asm: Source] |
| Plos Pathogens | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis[PubMed: PMC3715414] |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese[Pubmed: 24124699] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source] |
| Vaccine | On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment[Pubmed:25444792] |
| International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula[Doi: 10.1111] |
| Molecular Nutrition Food Research | Green?tea?polyphenol?epigallocatechin-3-gallate?improves?epithelial?barrier?function?by?inducing?the?productionof?antimicrobial?peptide?pBD-1?and?pBD-2?in?monolayers?of?porcine?intestinal?epithelial?IPEC-J2?cells.[Pubmed:26991948] |
| Respiratory Research | Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner.[pubmed:27655266] |
| Key Engineering Materials | The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture.[16629795] |
| Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique[Pubmed:29374283] |
| Frontiers in Microbiology | Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense[Pubmed:29997590] |
| Journal of Dermatological?Science | Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis[Pubmed:29496360] |
| International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase?…[Pubmed: 30342347] |
| 拉脫維亞科學院院刊 | Influence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines[] |
| Veterinary?Research | Bovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureus[Pubmed: 30975214] |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients[] |
| Veterinary Research | Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection[Pubmed: 31221216] |
| Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport[33375218] |
| Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus[34362937] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?…[Pubmed:35687771] |