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血管生成素2(ANGPT2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Angiopoietin 2 (ANGPT2)

ANG2; AGPT2

  • 血管生成素2(ANGPT2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 血管生成素2(ANGPT2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)血管生成素2(ANGPT2)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種：Bos taurus; Bovine (Cattle，牛)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA009Bo01血管生成素2(ANGPT2)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA009Bo01血管生成素2(ANGPT2)多克隆抗體WB; IHC; ICC; IP.
KSA009Bo01血管生成素2(ANGPT2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志參考文獻(xiàn)
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Sci Rep.?Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis.[pubmed:28276491]
PeerJ?The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker[pubmed:28584715]
Cellular Physiology and BiochemistryAngiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats[pubmed:28848201]
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Anticancer?ResearchOligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway[Pubmed: 30711932]
Comparative MedicineCharacterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female?…[Pubmed: 31526432]
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Scientific ReportsAssociation between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea[Pubmed: 31882827]
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