血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Angiotensinogen (AGT)
ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
- 編號(hào)KSA797Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.78-50ng/mL
- 可適靈敏度0.35ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對血管緊張素原(AGT)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA797Ra61 | 血管緊張素原(AGT)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA797Ra01 | 血管緊張素原(AGT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA797Ra61 | 血管緊張素原(AGT)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA797Ra01 | 血管緊張素原(AGT)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA797Ra71 | 血管緊張素原(AGT)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA797Ra81 | 血管緊張素原(AGT)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA797Ra25 | 血管緊張素原(AGT)單克隆抗體 | ICC/IF |
| MAA797Ra21 | 血管緊張素原(AGT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA797Ra22 | 血管緊張素原(AGT)單克隆抗體 | WB |
| MAA797Ra23 | 血管緊張素原(AGT)單克隆抗體 | WB |
| MAA797Ra24 | 血管緊張素原(AGT)單克隆抗體 | WB; IHC |
| MAA797Ra27 | 血管緊張素原(AGT)單克隆抗體 | IHC |
| MAA797Ra26 | 血管緊張素原(AGT)單克隆抗體 | WB; ICC/IF |
| FAA797Ra02 | 抗血管緊張素原(AGT)單克隆抗體 | FCM |
| SEA797Ra | 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA797Ra | 血管緊張素原(AGT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA797Ra01 | 血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Transplant International | Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients?[Wiley: source] |
| Amyloid | The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients[PubMed: 22320202] |
| Pediatric Mephrology | Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension[PubMed: PMC3661928] |
| Acta Paediatrica | Urinary angiotensinogen as a novel marker of obstructive nephropathy in children[Pubmed: 23772991] |
| Journal of the Renin-Angiotensin-Aldosterone System | Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia[Sagepub: Source] |
| Pediatric Nephrology | MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY[Oxfordjournals:Source] |
| journal of human hypertension | Relationship between blood pressure var[PubMed: 26223347] |
| PLoS One | Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis[PubMed: 26098644] |
| MKA | Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa[Index.Php: Art] |
| Journal of Human Hypertension | Relationship between blood pressure variability and renal activity of the renin-angiotensin system.[pubmed:26223347] |
| Acta Pharmacol Sin. | Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells.[pubmed:27890915] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| Life?Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity[Pubmed:30036488] |
| American Journal of Physiology-Renal?Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats[Pubmed:29846109] |
| Methods in Molecular Biology | Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia[Pubmed: 31069778] |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans[33407555] |
| Hypertens Res | Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents[33568792] |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy?\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/|??\catenin signaling[34057312] |
| Cancer Cell Int | A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer[34215253] |